Sample Journal Article Record
Accession Number: 9 7 2 9 2 5 0 5
Title: A spontaneous point mutation in the human T-cell leukemia virus type 1 Px gene leads to
expression of a novel doubly spliced Px-mRNA that encodes a 25-kD, amino-terminal deleted rex
p r o t e i n
Author(s): Orita S; Kobayashi H; Saiga A; Kubota R; Osame M; Igarashi H
Author Affiliation: Shionogi Inst. Med. Sci., 2-5-1 Mishima, Settsu-shi, Osaka 566, JAP
Bibliographic Information: DNA and Cell Biology 13 (4). 1994. 353-364.
ISSN: 1 0 4 4 - 5 4 9 8
Publication Year: 1 9 9 4
Language: E N G L I S H
Journal Announcement: Biological Abstracts Vol. 098 Iss. 001 Ref. 014150
Abstract: Primary RNA transcripts of the human T-cell leukemia virus type 1 (HTLV-1) are processed into
mature MRNA by a complex series of splicing events. In this paper, we report the finding of a novel
doubly spliced Px MRNA in two out of eight HTLV-1-infected cell lines and in one out of 13
peripheral blood mononuclear cells from HTLV-1-infected individuals. The second splicing for this
novel Px MRNA is different from that for the known doubly spliced Px MRNA. A novel acceptor site
in this splicing was generated by a single point mutation (G to A) at nucleotide 7,337 of the Px gene.
This MRNA contained a complete open reading frame that encodes an amino-terminal truncated
p27rex protein with 189 amino acids. A new 25-Kd protein was detected in the cell lines expressing
the novel Px MRNA by an antibody against the carboxy-terminal peptide of p27rex and was termed
p25rex. Although the function of p25rex is not clear, we clarified that p25rex is a cytoplasmic phos-
phoprotein and its function is different from the transcriptional regulator function of p27rex. The
possibility that the mutated virus is replicable only in cells coinfected with the wild type HTLV-1
may explain why the incidence of the mutants observed here is low.
Added Keywords: RESEARCH ARTICLE; MESSENGER RNA; SPLICING; P25REX; CYTOPLASMIC
P H O S P H O P R O T E I N
Major Concept Codes: *15006 Blood, Blood-Forming Organs and Body Fluids-Blood, Lymphatic and
Reticuloendothelial Pathologies
*15008 Blood, Blood-Forming Organs and Body Fluids-Lymphatic Tissue and Reticuloendothelial
System
*24010 Neoplasms and Neoplastic Agents-Blood and Reticuloendothelial Neoplasms
*31500 Genetics of Bacteria and Viruses
*33506 Virology-Animal Host Viruses
*36006 Medical and Clinical Microbiology-Virology
Minor Concept Codes: 10062 Biochemical Studies-Nucleic Acids, Purines and Pyrimidines
10064 Biochemical Studies-Proteins, Peptides and Amino Acids
Biosystematic Codes: 02623 Retroviridae (1993- )
86215 Hominidae
Super Taxonomic Groups: Microorganisms; Viruses; Animals; Chordates; Vertebrates; Mammals;
Primates; Humans
S a m p l e R e c o r d s
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S a m p l e R e c o r d s
