(p<0.01).Blood gas changes: The air pressure was decreased to the equivalent of 7500m and
maintained for thirty minutes. Blood gas samples were then quickly obtained. The PO2 of the
control group was 31.50±8.69mmHg, while in the big dosage group, moderate dosage group and
small dosage group it was 47.15±11.52mmHg, 42.36±9.55, 36.33±9.33, respectively. There was
significant difference between treatment groups and the control group (p<0.01). The SO2 of the
control group was 45.68±11.2568%, while in big dosage group, moderate dosage group and small
dosage group it was 20±8.56%, 60.21±10.58% and 55.21±10.58%, respectively. There was a
significant difference between the treatment groups and the control group (p<0.01). Results: The
Bos mutus Przewalski blood could significantly raise the survival ratio of rats while improving
the PO2, SO2 effectively; PCO2 and PH were not affected. Conclusion: This indicates that Bos
mutus Przewalski blood has anti-hypoxic activity.
216.
COMPARISON OF ENOS EXPRESSION IN THE LUNGS OF WISTAR RATS AND
QINGZANG PLATEU PIKAS. Jin Si
1
, Ye Shi-Qiao
1
, Wang Tao
1
, Wang Di-Xin
1
, Wu Tian-Yi
2
,
Sun Bing-Yong
3
. Department of pathophysiology, Tongji Medical College, Huazhong University
of Science 
14
78; Techn
1
, Institute of High Altitude Medical Science of Qinghai Provicine,
Xining,China
2
, Department of Pathophysiology,The Third Military Medical
University,Chongqing
3
.
NO plays an important role in hypoxic pulmonary responses, including hypoxic pulmonary
vasoconstriction (HPV) and hypoxic bronchial-dilation (HBD). To further find clues about its
role in hypoxia adaptation of high altitude animals, a comparison study of endothelial nitric oxide
syntheses (eNOS) expression between wistar rats and Qingzang Plateau Pikas were performed.
The lungs of 6 wistar rats(obtained from XiNing, about an altitude of 2200m,Qinghai Province,
China) and 8 pikas(obtained from Guoluo, about an altitude of 3800m,Qinghai Province, China,)
were fixed with 4% polyformaldehyde and an immunohistochemistry were followed(endothelial
nitric oxide synthase primary antibody were purchased from Santa Cruz). The data were analyzed
by Image-pro-plus 3.0 software (Media Cybernetics. USA). Compared with wistar rats, pikas
expressed a much lower value of eNOS in pulmonary artery endothelial cells; nevertheless, they
expressed a much higher value of eNOS in airway epithelial cells. We thought a lower eNOS
expression level in pulmonary artery endothelial cells may be a result of lower shear stress caused
by a blunt HPV in pika, while a higher NO level in airway may be a compensation of a decreased
O2 level in high altitude.
217.
COMPARATION OF A MAXIMAL TEST AT 4000 M IN HYPOBARIC CHAMBER VS 4000
M EN NATURAL CONDITION AT JAMA PATH CHILE. . Juan Silva
1
, Ana Wall
1
, Marcela
Moscoso
1
, Angélica Olivares
2
, Paulina Spencer
3
, José Rojas
1
, Gustavo Contreras
4
. University of
Antofagasta
1
, University of Antofagasta
2
, University of Antofagasta
3
, Asociación Chilena de
Seguridad
4
.
The special geographic conditions in the north of Chile and the neighbor countries the
exposure to intermittent hypoxia occurs to occupational, sports and tourism level. In the sports
context it has been developed intermittent hypoxia exposure protocols in hypobaric chambers in
order to optimize the acclimatization of the persons to improve the hematological parameters to
obtain better results in competition with altiplanic countries. Actually the hypobaric chamber
(HC) tests are not standardized, then it is not possible assert that the physiological demand in a
simulated altitude is similar to the same test in the natural geographic condition. Trying to solve
this problem, physiological parameters non invasive in 27 college students 20 ± 1 year old were
checked. Students performed sub maximal test in cycloergometer simulating 4000 meters over
the sea level in the Hypobaric Chamber (HC) and at 4000 m. in the Jama Path (JP) in the north of
Chile. Mean values comparing HC vs. JP were: heart rate 157 beat/ min vs. 151 beat/min (p 0.1);
% hemoglobin saturation was 75% vs. 81% (p < 0.05) and the systolic pressure was 141 mmHg
vs. 151 mmHg (p < 0.05). The subjective perception of the effort following the Borg scale was
hard effort intensity (4.9) in HC and moderate effort intensity (4.1) in JP. Heart rate and the
