The aim of this study was to evaluate the management and results of newborns during
operation in moderate altitude_2260m_. The authors report on 182 newborns with congenital a
rectal malformation at an altitude of 2260m between 1986 and 2002. There were 180 male and 2
female, mean age was 4h to 30d. Immediate operations were performed on them. The blood
routine, blood gas and temperature in neonatal surgery were examined. Their general results,
mortality, morbidity of complications were also reviewed. The results were compared with that
with same patients (Children's Hospital of Shanxi Province) at sea level : 1. Mean value of the
hemoglobin and red cell in moderate altitude (19.1±2.5g/l, 6.2±1.3_1012 /l ) are higher than that
in sea level (18.1±0.3g/l, 4.3±0.7_1012/l ), but SaO2 (arterialized blood) and temperature
(65.6±3.9_, 32.8±2.4 ) is lower than that at sea level (90.1±6.2_, 36.5±1.2 ), P<0.001. 2. SaO2
(arterialized blood), hemoglobin, red cell and temperature of preoperative were higher than those
in postoperative (P<0.001). 3. At moderate altitude, mortality is 14.3%, morbidity of pneumonia
is 58.2%, scleredemais 73.1%, incision infection is 46.2%, and this is significantly higher than
that of the Shanxi (p <0.001). The authors conclude these results during operation were mainly
caused by cold climate and hypoxia at moderate altitude .So it is very important for the neonatal
surgery patients to be warmed and oxygenic in there. Give them a better potential nutrition and
body acid-base equilibrium may be decreasing their mortality and complications.
187.
EFFECT OF HYPOXIA ON MEMBRANE TRANSLOCATION LEVEL OF PKCS ISOFORMS
IN NEURONS. Yanming Qu
1
, Junfa Li
1
, Pengyu Zu
2
, Xiaodong Zhang
3
, Qunyuan Xu
2
. Dept. of
Neurobiology, Capital University of Medical Sciences, Beijing, China
1
, Dept. of Neurobiology,
Capital University of Medical Sciences,Beijing, China
2
, Dept.of human Anatomy, Histology and
embryology, Peking Union Medical College, Beijing
3
.
Hypoxic preconditioning (HP) is an intrinsic adaptive condition that results in tolerance in
different organs, such as brain. Elucidating the mechanism for HP and identifying drugs that
activate critical mediator of this process have the potential to improve the prognosis of ischemic
or hypoxic diseases. Recent studies have suggested a critical role for protein kinase C (PKCs) in
the development of cerebral HP. In this study, we have used the models of hypoxic stimulated
neuroblastoma cells (SH-SY5Y) to determine which isoform takes part in the process of HP-
induced neural protection by evaluating the dynamic redistribution of PKCs isozymes. The
content of PKCs in cytosol or particulate fractions of cultured SH-SY5Y cells were analyzed
semi-quantitatively by SDS-PAGE, Western-blot and Gel Doc imagine system. It was found that
the decreased protein level of cPKC, , nPKC, aPKC in cytosol coordinated with the increment in
particulate, which phenomenon was regarded as membrane translocation or an indicator of PKCs
activation, in response to hypoxic exposure time_0.5, 2, 4, 6, 8, 12 and 24hours) and the
significant membrane translocation of cPKC , nPKC and aPKC were found after 2 hours of
hypoxic exposure (P<0.001). However, there were no significant changes in membrane
translocation of the rest 8 PKCs isoforms. In addition, we have used immunocyto-chemistry
technique and found that nPKC translocated from cytosol into the nucleus_during hypoxic
exposure in SH-SY5Y cells, but nPKC still mainly located in the cytosol. These results
suggested that cPKC , nPKC , and aPKC may play an important role in the process of cerebral
hypoxic preconditioning.
188.
INVESTIGATION OF THE CORRELATON OF INSERTION/DELETION POLYMORPHISM
OF THE ANGIOTENSIN CONVERTING ENZYME GENE AND PREVALENCE OF AMS.
Hu Quan-Zhong
1
, Sun Ning-Ling
2
, Wu Shi-Zheng
3
. Department of neurology;Qinghai Province
People Hospital; Qinghai, Department of cardiology, Beijing University People Hospital;Beijing,
Department of Neurology; Qinghai Province People Hospital;Qinghai.
To investigate the relationship among Insertion/Deletion polymorphism of the angiotensin
converting enzyme (ACE) gene and arterial elasticity (AE) parameters in normal men on acute
ascension to high altitude and the prevalence of acute mountain sickness (AMS). Methods: 30
subjects who had lived at sea-level were included in our research. All subjects underwent non-
