146.
RETROSPECTIVE INVESTIGATION OF INPATIENTS WITH CARDIOVASCULAR
DISEASES IN TIBETAN PEOPLE'S HOSPITAL OVER THE LAST SEVENTEEN YEARS.
Gesang Luobu
1
, Gen Wei-Jun
1
, Cen Yon
1
, Li Kui
1
, Zhang Lin-Jun
1
, Ren Dan
1
, Zhuoma Ciren
1
,
Pingcuo Zhaxi
1
, Zhuang Lan-Ping
1
. Tibetan People's Hospital, Tibet Research Institute of
Mountain Sickness and Cardiovascular Disease,
1
.
Cardiovascular diseases (CVD) are common disorders in the rugged environment of Tibet
(above 3360m). The aim of this study was to summarize the general situation, prognosis and
trend of CVD in inpatients in Lhasa over time. The data of 3,290 patients with CVD hospitalized
in Tibetan People's Hospital from 1986 to 2003 were selected. Of these patients, 1,639 were male
(49.8%) and 2,651 were female (50.2%). Subjects were divided into three groups according to
admitting period: first group (1986-1991), second group (1992-1996) and third group (1997-
2003). We compared the category, outcome and mortality of CVD among the three groups. The
age ranged from 17 to 82 yrs with a mean 50 yrs. 79% of these patients were Tibetan and 20%
were Han, only 1% represented other ethnic groups. More than 30% of these patients were over
60 years old. The types of CVD were as follows: hypertension (47.7%), arrythmia (15.5%),
rheumatic heart disease (8.9%), and coronary heart disease (6.9%). From the first to the third
group, the proportion of patients aged more than 60 years was increased from 23.1% to 36.1%.
The rates of hypertension, coronary heart disease and arrythmia increased, especially coronary
heart disease which increased from 4.4% to 9.1%, p<0.001. The rate of rheumatic heart disease
fell from 13.0% to 4.2% (p<0.001). The mortality rates of CVD from the first to third group were
2.51%, 3.38% and 2.05%, respectively. Although there was a trend of decreasing mortality, there
was no significant difference. In the first group, the most common cause of death was rheumatic
heart disease followed by hypertension. In the third group, the leading cause of death was
coronary heart disease followed by hypertension. In conclusion, over the past 18 years, the
incidence of CVD increased as did the proportion of elderly patients with CVD. The proportion
of hypertension remained the highest among inpatients with CVD.
147.
THE ANTI-NECROSIS EFFECT OF HYPOXIC PRECONDITIONING IN PC12 CELLS .
Zimin Ma
1
, Aishi Ding
1
, Liying Wu
1
, Yan Wu
1
, Tong Zhao
1
, Fuzhuang Wang
1
, Ming Fan
1
.
Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Beijing,
China
1
.
Objective: To study the effect of hypoxic preconditioning (HP) on the necrosis of PC12 cells
following acute hypoxic and the mechanism of anti-necrosis effect. Methods: Acute hypoxia
(AH) was induced by culturing PC12 cells under anoxia condition of 5% CO2, 95% N2 for 24
hours. For HP exposure, PC12 cells were cultured with DMEM medium (contain 1g/L glucose)
under the condition of 10% O2, 5% CO2, 85% N2 for 48h. The rate of released lactate
dehydrogenase (LDH) from cells was employed to assess the extent of cell injury. Results:
Hypoxia tolerance of PC12 cells was significantly augmented after 48 hours HP exposure, by
which aldose reductase (AR) expression was up-regulated and sorbitol products increased
remarkably. After exposure to (AH) for 24h, the rate of LDH release was 32.0%±6.1% in HP
treated cells and 50.2%±5.9% in AH group. Pretreatment with quinidine to block the intracellular
sorbitol release or berberine to inhibit AR activity resulted in increase of rate of LDH release
significantly. Conclusions: Sorbitol may play an important role in anti-necrosis effect of
preconditioning hypoxia in PC12 cells. The work was supported by key grant of Natural Sciences
Foundation of China (30393130)
148.
MUSCLE TISSUE ADAPTATIONS TO NORMOBARIC INTERMITTENT HYPOXIC
EXPOSURE. Irina Mankovskaya
1
, Katherine Lyabakh
2
, Bronislav Gavenauskas
1
, Olga Gonchar
1
,
Valentina Nosar
1
, Ada Nazarenko
1
, Katherine Rozova
1
. Bogomolets Institute of Physiology
1
,
Glushkov Institute of Cybernetics
2
.
