Hospital, Xining,, Qinghai; P.R. China
1
, Qinghai Provincial People's Hospital, Xining, Qinghai;
P.R. China
2
.
To study characteristics of breathing disorders during sleep in normal subjects ascending to
the high altitude (4850 meters) and the effects of Yixinkangtai and Yixiancuoan on these
disorders. Methods 18 subjects were randomly divided into 3 groups (n=6 each group): those who
received Yixinkangtai, those who received Yixiancuoan and control. They went to high altitude
after taking the medicines for 25 days. Their sleep patterns and breathing were observed. Result
showed that phase I and II of non-rapid eye movement sleep (NREM) shortened, but phase III
and IV of non-rapid eye movement sleep and rapid eye movement sleep (REM) was prolonged in
the medicine treatment group. In the control group, phase I and II of non-rapid eye movement
sleep (NREM) was prolonged while phase III and IV of non-rapid eye movement sleep and rapid
eye movement sleep (REM) shortened. There were differences between both groups (p<0.01).
The hourly number and average time of sleep apneas (SA) and periodic breathing (PB) decreased
in the medicine treatment group (p<0.05). Conclusion: Yixinkangtai and Yixiancuoan can
improve the sleep-patterns and breathing disorders of subjects ascending to the high altitude and
can control central sleep apnea (CSA).
109.
PRIMARY SCREENING FOR HYPOXIA-RELATED GENES. Xiaotang Jing
1
, Wenhong Fan
1
,
Yan Wu
1
, Yingzhong Yang
2
, Rili Ge
2
, Ming Fan
1
. Department of Brain Protection and Plasticity,
Institute of Basic Medical Sciences, Beijing, China
1
, Qinghai High Altitude Medical Institute,
Xining, China
2
.
Objective: To identify the hypoxia-related genes from nova genes that we got from human
brain cDNA library by means of SSH. Methods: P19 cells were treated with 3% and 10% O2
(hypoxia) for 24 hours After planted in a-MEM for one day, then total RNA were extracted by
using the trizol method. For in vivo study, mice were divided into 3 groups as follows: acute
anoxia, intermittent hypoxia and control. Total RNA was extracted from hippocampus, pituitary,
cortex and cerebellum. The mRNA expression of these genes was detected with semi-quantitative
reverse transcriptase polymerase chain reaction (RT-PCR). Results: Three genes were
significantly changed folowing hypoxic treatment. mKIAA1599, a copine family protein with a
von willebrand/integrin A domains, which could not be detected under 3% oxygen concentration
in P19 cells. Interestingly, its expression was increased in cortex and cerebellum but was reduced
in hippocampus in vivo. The second gene is mouse BRI3, a membrane integral protein, were
reduced both in P19 cells and hypoxic animals. Finally, one expression sequence tag (EST),
which has no documents update, was obviously increased under 3% O2 condition in P19 cells.
Other detected genes have no significant alterations in our study. Conclusions: Results suggest
that our model is efficient for screening of hypoxia responsive genes at the transcriptional level,
and mKIAA1599, BRI3 and one EST may be involved in body response to hypoxia The work
was supported by key grant of Natural Sciences Foundation of China (30393130)
110.
INVESTIGATION OF CHANGES IN HAPTOGLOBIN LEVELS OF PATIENTS WITH
HAPC. Zhou Jing-Ping
1
, Peng Hai
1
, Qi Zhao
1
, Gu Sun-Qing
1
, Liu Lan
1
. Department of immunity,
Qinghai Provincial People's Hospital, Xining,, Qinghai, P.R.
1
.
High altitude pulmonary polycythemia (HAPC) is a disease of chronic exposure to high
altitude. The purpose of this paper was to study variations in haptoglobin (HPT) levels of patients
with HAPC. 42 HAPC and 50 normal subjects at high altitude (4750m) and 50 normal subjects
from lowland were tested by dispersionnephelometer assay with special protein analysis
instrument (Benckman-Array360). The results showed that HPT levels of the three groups were
0.58±0.42g/L, 0.75±0.35g/L and 0.81±0.41g/L respectively. The HPT on the patients with HAPC
was lower than that of normal subjects at high altitude and at lowland (P<0.05±0.01). It has been
suggested in some publications that HPT is an important predictor of anemia. HPT decreased in
hemolytic anemia. Lower HPT levels correlated with the greater degrees of hemolysis. But the
