after hypoxia and conbined exercise in both ventricles. CSA (_m2)of and CD (number/mm2) in
rat gastrocnemius muscle
CSA
Gr
oup
n
Type
Type
CD
C/F
ratio
8
2947±2
35
2391±2
23
599±42
1.62±0.
12
6
2252±3
62*
2104±1
60*
661±50
*
1.63±0.
18
6
3262±1
78*#
2331±2
86#
757±34
*#
2.17±0.
12*#
*p<0.05 vs control; # p<0.05 vs hypoxia CSA (_m2) of myocardial fiber and CD
(number/mm2)
Left ventricle
Right ventricle
Gr
oup
CSA
CD
C/F
ratio
CSA
CD
C/F
ratio
547±
67
2192±3
46
1.14±0.
07
499±
79
2156±2
33
1.20±0.
15
545±
135
2585±2
83*
1.34±0.
18*
856±
77*
2047±2
60
1.61±0.
21*
568±
63
2909±1
47*#
1.61±0.
16*#
873±
104*
2812±3
84*#
2.12±0.
39*#
*p<0.05 vs control; # p<0.05 vs hypoxia
Conclusion: Exercise during hypoxic exposure can lead to capillary formation, which may
benefit acclimation to high altitude. (By grant of NSFC 39730190 and 30393131)
88.
CHRONIC HYPOXIA EXPOSURE INCREASES GLUCOSE UPTAKE IN SKELETAL
MUSCLE. Jiang Huang
1
, Yuqi Gao
1
. Department of Pathophysiology and High Altitude
Physiology, Third Military Medical University, Chong
1
.
Objective: To investivage the effects of acclimatization to hypobaric hypoxia on glucose
uptake in skeletal muscle. Methods: Wistar rats were divided into four groups randomly: Control
group H0, hypoxic 5 days H5, hypoxic 15 days H15, hypoxic 30 days H30. Hypoxic groups were
exposed to hypobaric hypoxia (simulated 5000m) for 5, 15, 30 days respectively, 23h/d. The H0
group was raised at sea level. Skeletal muscle from hind limb was collected. 3H-D-glucose was
used to assay the glucose uptake rate GUR in vitro. The level of glucose and glycogen were
assayed by enzyme methods. The intracellular and plasma membrane of skeletal muscle were
separated by discontinuous sugar gradient 25, 35%. The maximal binding sites Bmax and Kd of
glucose transporters on the membranes were analyzed by scatchard. Results: Compared with H0,
the GUR of skeletal muscle in H5 was increased, the glucose level of H5 did not increase
significantly, and the glycogen level was reduced in H5. The Bmax of glucose transporters in
plasma membrane was also increased while that in the intracellular membrane was decreased.
Compared with H5, the GUR in H15 or H30 was reduced, GUR in H30 was not higher than that
in H15, and the glucose and glycogen level of H15 or H30 were significantly increased. The
Bmax of glucose transporters in plasma membrane increased in both of H15 and H30. Kd of the
glucose transporters remained unchanged in the hypoxic groups. Conclusion: Chronic hypoxia
exposure promotes the translocation of glucose transporters in skeletal muscle and increases the
potential ability for glucose uptake, which may be one of the important mechanisms for
acclimatization to high altitude. (By grant of NSFC 39730190 and 30393131).
89.
ANALYSIS OF PHYSIOLOGICAL FACTORS RELATED TO AMS USING
MULTIVARIATE REGRESSION . Qingyuan Huang
1
, Yuqi Gao
1
, Fuyu Liu
1
, Xinbing Mu
1
,
