51.
SUPPRESSIVE EFFECTS OF HYPOXIA ON QUANTITY AND QUALITY OF
SPERMATOZOA IN RATS. Yuqi Gao
1
, Weigong Liao
1
, Mingchun Cai
1
, Yi Wu
1
, Jian Chen
1
.
Department of Pathophysiology and High Altitude Physiology, Third Military Medical
University, Chong
1
.
Objective: To explore the effects of hypobaric hypoxia on the quantity and quality of
spermatozoa in rats. Methods: Male Wistar rats were randomly divided into four groups: control
group, 5d hypoxic group, 15d hypoxic group and 30d hypoxic group. Control group was raised at
sea level, hypoxic groups were raised in a hypobaric chamber simulating 5000m altitude for
5days, 15days and 30days respectively. The density, motility, and morphologic abnormality of
spermatozoa in epididymal cauda, and the lysophosphatidyl choline (LPC)-induced acrosome
reaction (AR) were examined. The acrosin activity of spermatozoa was also assessed. Results:
The spermatozoa density significantly decreased in 15d (1.86±0.28_107/100mg epididymal
tissue) and 30d (2.77±0.17_107/100mg epididymal tissue) hypoxic groups, compared with
control group (3.53±0.23_107/100mg epididymal tissue)(P<0.01). Motilities of epididymal
caudal spermatozoa decreased by approximately 50% in all three hypoxic groups(P<0.01,
compared with control group). Morphological abnormal spermatozoa increased by more than
50% in all three hypoxic groups(P<0.01, compared with control group). The LPC-induced AR
was suppressed by about 60% in all three hypoxic groups(P<0.01, compared with control group).
Acrosin activity decreased significantly after exposure to hypoxia for 5, 15, and 30days(P<0.01,
compared with control group). Conclusions: Hypobaric hypoxia suppresses the sperm quantity
and quality, as well as inhibits the acrosome reaction of rat spermatozoa. (By grant of NSFC
39730190 and 30393131).
52.
THE DIAGNOSIS AND TREATMENT OF ACUTE RESPIRATORY DISTRESS SYNDROME
AFTER OPEN-HEART SURGERY AT ALTITUDE. Bingren Gao
1
. Gansu Hospital of
cardiovascular diseases, The First Affiliated Hospital of Lanzhou Medical College
1
.
Objective: Discuss the mechanism and the early diagnosis and treatment of acute respiratory
distress syndrome (ARDS) after cardiopulmonary bypass (CPB) for heart diseases at high
altitude. Methods: Eighty-six patients with ARDS after open-heart operation received early
diagnosis and underwent mechanical ventilation. Meanwhile, they were treated with methods of
reducing pulmonary capillary wedge pressure (PCWP) and extravascular lung water (EVLW).
Result : many factors injuring lung function in cardiopulmonary bypass can result in ARDS, such
as systemic inflammatory response syndrome and pulmonary ischemic-reperfusion injury. With
ARDS combined with renal failure and poly-organ failure, five patients died. The hospital
mortality rate was 5.7%. The others recovered and in long-term follow-up there are no sequelae
of lung injury. Conclusion: Modern treatment of ARDS should treat systemic inflammatory
response syndrome and acute respiratory failure effectively and at the same time cure multiple
systems organ failure (MSOF). Machine ventilation with optimal PEEP and depressing capillary
hydraulic pressure and increasing cardiac muscle contractility and depressing the preload and
afterload of the heart are mostly effective therapy of ARDS .
53.
ERYTHROPOIETIN: PROTECTING AGAINST TISSUE INJURY. Max Gassmann
1
. Inst. of
Veterinary Physiology, University of Zurich
1
.
Precise regulation of erythropoiesis is crucial since severe elevation of red blood cell number
is associated with hypertension and thromboembolism resulting in severe cardiovascular
complications. Erythropoietin (Epo) controls erythropoieses in an oxygen-dependent manner. To
test the impact of hypoxia-independent expression of Epo, we generated a transgenic (tg) mouse
line termed tg6 that, due to constitutive expression of human Epo, reached hematocrit values of
0.8 to 0.9 without alteration of blood pressure, heart rate or cardiac output. Extramedullary
erythropoieses occurred in the tg spleen leading to splenomegaly. Upon splenectomy, hematocrit
