41.
ISOLATION AND IDENTIFICATION OF DIFFERENTIALLY EXPRESSED PROTEINS
FROM HYPOBARIC HYPOXIC DELAYED PRECONDITIONING OF HIPPOCAMPUS IN
MICE. Youming Fan
1
, Yuqi Gao
1
, Jiang Huang
1
, Jiang Chen
1
, Mingchun Cai
1
. Department of
Pathophysiology and High Altitude Physiology, Third Military Medical University, Chong
1
.
To explore the differentially expressed proteins between hypoxic preconditioning and normal
mice hippocampus. Methods: Hypobaric hypoxic delayed preconditioning was performed in mice
and hippocampal proteins were obtained by a series of abstraction with lysis solution. SDS-PAGE
and isoelectric focusing were performed. After the electrophoresis, the proteins in the 2-DE gels
were visualized. The gels were scanned, and the images were processed with PDQuest software.
Differential proteins were exactly excised from the gels. The peptides were isolated and sent for
MALDI-TOF-MS testing. Database searching was performed using peptide masses obtained from
MALDI-TOF-MS. Results: Averages of 481±38 and 477±21 protein spots were detected in
control gels and preconditioning gels, respectively. 169±6 protein spots were matched between
these two types of gels. Among matched spots, while the quantities of 21±12 spots in control gels
increased by above 2 times than in preconditioning, the quantities of 33±10 spots in
preconditioning gels doubled over control. The correlation coefficient between these two patterns
was 0.7748±0.0267. The correlation coefficient of 12 spots in preconditioning gels significantly
increased compared with the control (P<0.05). Among 12 spots excised from the gels, perfect
peptide mass fingerprinting spectrum of 8 spots was acquired. The results showed one of them
was fructose biphosphate aldolase; three of them could not be matched, which might represent
new proteins. Although four others matched some peptides, we could not confirm what protein it
is. They may be homologous genes. Conclusion_Part of proteins, for example fructose
biphosphate aldolase, have been differentially expressed in hypobaric hypoxic delayed
preconditioning hippocampus of mice. They could help to explain the molecular mechanism of
delayed preconditioning. (By grant of NSFC 39730190 and 30393131)
42.
A STUDY OF HEMORHEOLOGY IN NORMAL SUBJECTS ASCENDING TO HIGH
ALTITUDE. Meng Fang, Feng Jian-Ming, Li Xiao-Wei, Shi Qing-Wu, Fan Xiu-Ru, Sun Zhi-
Xing. Department of Blood, The People's Hospital of Qinghai, Qinghai.
To study the changes of hemorheology of normal subjects ascending to the high altitude.
Method: We used the rotary hemorheology analysis instrument (LBY-N6) produced by Beiljing
Prison Group. The samples were divided into the Germu group (2800m, n=28) and Tuotuo River
group (4700m, n=28). Result: The titer of whole blood viscosity, plasma viscosity, reduced blood
viscosity and hematocrit were 13.66±3.61mPa·S, 1.81+0.22 mPa·S, 24.86±4.67 mPa·S and
50.31+5.49%, respectively, in the Germu group and 20.56+4.10 mPa·S, 2.04+0.29 mPa·S,
29.56+3.91 mPa·S and 64.79+5.32%, respectively, in Tuotuo River group. There was a
significant difference between all parameters in both groups (P<0.001). Under low shear rate and
high shear rate, the whole blood viscosity and the reduced blood viscosity were increased,
indirectly suggesting increased aggregability and decreased deformation. Conclusion: The results
showed increased viscosity with increasing altitude.
43.
RIGHT TEMPORAL EEG PREDICTS OCCURRENCE OF ACUTE MOUNTAIN SICKNESS .
Berend Feddersen
1
, Harald Ausserer
1
, Pritam Neupane
2
, Florian Thanbichler
1
, Martin Dugas
3
,
Robb Waanders
4
, Soheyl Noachtar
1
. Department of Neurology, Klinikum Grosshadern,
University of Munich, Germany
1
, Human Development and Community Services, Kathamdu,
Nepal
2
, Department of Medical Informatics and Biometrics; University of Munich, Germany
3
,
Department of Neuropsychiatry, Landeskrankenhaus Rankweil, Austria
4
.
We prospectively investigated the effects of high altitude on brain function measured by
electroencephalography (EEG) and transcranial doppler sonography (TCD) in relation to end-
expiratory CO2 (EtCO2), peripheral O2 saturation (SaO2) and symptoms of acute mountain
sickness (AMS). The studies were performed during a common trekking route at Khumbu Himal,
