37.
SERUM VEGF LEVELS IN PATIENTS WITH HAPC. Yang Fa-Man
1
, Li Zhan-Quan
1
, Wei
Hua
1
, Liu Ji
1
, Liu Li
1
, Wang Yuan-Jun
1
, Li Rong
2
. Qinghai Medical College Hospital, Xining,
Qinghai, P.R. China
1
, Qinghai Medical College Hospital, Xining, Qinghai, P.R. China
2
.
This study aimed to investigate the effect of Vascular Endothelial Growth Factor (VEGF) in
the pathogenesis and development of high altitude polycythemia (HAPC). Methods: The
concentration of serum VEGF in a group of patients living at 4300M with HAPC and a group
controls were detected by SPS-ELISA. The control group was divided into 3 groups, e.g. healthy
natives, immigrants (further divided it into 2 subgroups: moved into higher altitude in a shorter
time <14 days and the group of longer time 1 year living at plateau) and healthy natives living in
Xining. Results: Serum VEGF in patients with HAPC was significantly higher than that of the
control group (p<0.001). Moreover, serum VEGF in subgroup of moved into higher altitude in a
shorter time <14 days was distinctly higher than the group of HAPC, healthy natives group,
immigrants group and healthy natives living in Xining (P<0.001). There was no significant
difference among all three control groups. The levels of Hb, Ret and Hct in the HAPC group were
dramatically higher than that of three control groups. Conclusions: 1.The transcription and
expression of hypoxia-reactivw VEGF gene is instantly increased after entering into high altitude
hypoxia environment in a short time. 2. The levels of VEGF and Hb in immigrants group were
decreased or returned to normal level after adaptation to the hypoxia environment in the
immigrants group. 3. HAPC could stimulate the bone marrow erythropoeitic system to cause
constant hyperplasia from a lack of oxygen. Proliferation of erythropoeitic system may cause
changes in blood density, viscosity, higher conglomeration and coagulation and ultimately
aggravate hypoxemia. In addition, the lack of oxygen in cells may cause increased VEGF
expression.
38.
EXPRESSION OF TRANSFERRIN RECEPTOR IN HIGH ALTITUDE POLYCYTHEMIA.
Yang Fa-Man
1
, Jia Nai-Yong
1
, Li Zhan-Quan
1
, Wei Hua
1
, Liu Ji
2
, Liu Li
1
, Wang Yuan-Jun
1
, Li
Rong
3
. Qinghai Medical College Hospital, Xining, Qinghai, P.R. China.
1
, Qinghai Medical
College Hospital, Xining, Qinghai, P.R. China.
2
, Qinghai Medical College Hospital, Xining,
Qinghai, P.R. China.
3
.
The Pathogenesis of High Altitude Polythemia HAPC is thought to be directly connected
with environmental factors such as altitude, hypoxia, low atmospheric pressure, and so on. A
great deal of data have shown that it is an erythroblast proliferative disease, and closely related
with erythropoietin EPO), its receptor EPOR, and TPO, IL-3 and IL-6. This study explored the
relation between iron metabolism of hematopoiesis and HAPC by determining the concentration
of serum sTfR and SF. Methods: The concentration of serum sTfR and SF in patients, natives and
immigrants living in high altitude area with HAPC, were assessed by SPS-ELISA and healthy
natives living in Xining City were selected as controls. Results: The concentrations of serum
sTfR in patients with HAPC were significantly higher than that in high altitude natives and
immigrants (F=34.22 P<0.001. There was also a significant difference in serum sTfR among the
healthy Xining natives, high altitude natives and immigrants_F= 34.22 P<0.001. There were
positive correlations among serum sTfR, Hb, Hct and Ret, and negative correlation between
serum sTfR and TF_-5.93 P<0.001. There was no correlation between serum sTfR and SF.
Conclusion: Serum sTfR in patients with HAPC was increased and was positively correlated with
Hb, Hct, Ret and fR. Serum sTfR reflects iron metabolism in erythron, so it was used as the
quantitative analysis method to determine functional iron concentration in cells. The level of
serum sTfR reflects the proliferation of erythroblast in bone marrow when iron metabolism is
normal; therefore, it was used as the method to quantitatively analyze proliferation of erythroblast
in bone marrow.
