the tissues were obtained from the brain. HIF-1 were harvested to apply the RT-PCR and the
forward and backward primers including the sticking ending for ligase designed carefully and
optimally with highly conserved rabbit HIF genes with the data from the GENBANK. Then the
HIF was amplified and inserted to the PET vectors (Invitrogen) PET-30a electro transported to
the E. Coil BL-21 (DE-3) (Invitrogen) for inducing by IPTG and expressing the protein of the
HIF. We lysed the cell for total cell protein for the protein SDS-PAGE electrophoresis. Then the
gels stained by Coomassie blue and imaged with the white transilluminator (UVP). The
characteristic and thick lanes appeared on the gel and the weight was about 20KD calibrating
with the marker (SIGMA). Conclusion: The HIF-1 expressed and firstly observed on the SDS-
PAGE. Maybe for lifelong livings in the high altitude cause the HIF-1 expression increased in the
Plateau Pika and the deeply research for the quantity and quality compares should be done. The
work was funded by Natural Sceince Foundation of China (302600354)
305.
EXPERIMENTAL STUDY OF KIDNEY ERYTHROPOIETIN GENE EXPRESSION IN RATS
EXPOSED 2000M ALTITUDE BREATHING DIFFERENT ENRICHED OXYGEN . Qin Zhi-
Feng
1
, Chen Juan
1
, Xiao Hua-Jun
1
. Institute of Aviation Medicine, Air Force, Beijing, China
1
.
Acute altitude hypoxia and fulminating altitude hypoxia is the most harmful one of many
worse environmental factors for pilots. In modern aviation technology, although there is a fine
oxygen system, pilots often suffer from different hypoxia because of the loss of cabin pressure,
unfitness of mask, trouble with the oxygen system and so on. Scholars of many countries have
studied altitude hypoxia for a hundred years. With the development of molecular biological
technology, the pathological and physiological studies of hypoxia are pushed forward. The
purpose of this study is to observe the kidney erythropoietin gene expression in rats during
fulminating altitude hypoxia under different protections. Methods: 24 male Wistar rats were
divided randomly into 6 groups: The ground control group, the 3 000m control group, the
fulminating altitude hypoxia group, the group breathing 90% oxygen, the group breathing 100%
oxygen. They were exposed to 12 000m fulminating altitude hypoxia; in the meantime they
inhaled oxygen with different concentrations for 30 min. Then they were decapitated after
treatment and tissue samples of kidney were taken for reverse transcription polymerase chain
reaction (RT-PCR) and the mRNA expression changes of EPO gene were measured by semi-
quantitative RT-PCR. The results of EPO genes study show that the expression level of rat EPO
genes: the group breathing 50% enriched oxygen the fulminating altitude hypoxia group the
group breathing 100% oxygen the group breathing 90% enriched oxygen the 3 000m control
group the ground control group. Compared to the ground control group, the EPO gene expression
of rats in the group breathing 50% oxygen, the group breathing 100% oxygen and the fulminating
altitude hypoxia group increase obviously. The group breathing 50% oxygen increase most,
which is 3.1 times as much as the ground control group. The protective effect of breathing 100%
oxygen and breathing 90% oxygen for 12 000m altitude expose is equal to the 3 000m control
group.
306.
CARDIOPROTECTIVE EFFECTS OF INTERMITTENT HIGH ALTITUDE HYPOXIA
AGAINST ISCHEMIA/REPERFUSION INJURY IN RAT HEART. WeiZhong Zhu
1
, JianWen
Dong
1
, HaiFen Zhu
1
, HaiLei Ding
1
, ZhaoNian Zhou
1
. Laboratory of Hypoxic Cardiovascular
Physiology, Shanghai Institutes for Biological Science Chinese
1
.
Cardioprotective effects of intermittent high altitude hypoxia against ischemia/reperfusion
injury in a rat heart. The aim of this study was to investigate the mechanisms of cardioprotection
against ischemia/reperfusion-induced injury in rat hearts adapted under intermittent high altitude
hypoxia (IH). Methods: Adult male SD rats were put into the hypobaric chamber (simulated
5000m) for 6h daily, lasting 42 days. Isolated hearts were subjected to 30 min global ischemia
followed by 30 or 60 min reperfusion. Glibenclamide, 5-hydroxydecunoate or pinacidil were
administrated before ischemia. Cardiomyocytes [Ca2+]i were measured using a digital CCD. The
activation and translocation of PKC-_,_,_ isozymes, Bax and Bcl-2 were examined by Western
