arteries, Rho kinase inhibition (Y-27632 10-7M), decreased NE-induced contraction and [Ca2+]i
to a similar extent. By Western immunoblot LTH arteries, had 1.7-fold higher expression of both
total and phosphorylated CPI-17. Also, peak NE-induced MLC20 phosphorylation in normoxic
vessels occurred at ~ 20 sec, while in LTH vessels this was at 10 sec and by 15 sec
phosphorylated MLC20 had returned to basal level. Our data suggests that LTH-induced an
increase in Ca2+ sensitivity is regulated, in part, through the modulation of MLC20
phosphorylation via CPI-17. Also, although Rho kinase plays an important role in regulation of
NE-induced contraction and [Ca2+]i, it did not appear to play a major role in LTH-induced
changes (Supported by HD 03807).
302.
OUTLOOK OF TIBETAN MEDICINE ON ECOLOGY . Dan Zhe-Jia
1
, San Zhi-Jia
1
. Qinghai
Tibetan Medical College, Xining, China
1
.
Tibetan medicine is a science in a situation of special plateau which is created by Tibetan
people through fighting against diseases. What the important idea of it is the union of both
external and internal environment. Tibetan medicine believes that the Sa(dust), Chu(water),
me(fire), Rlung(wind) and Namkha(empty) are basic elements which constitutes all creatures and
the universe. The five elements play an important role in the existence and development of all the
creature and the universe. They make a close contact between the human and nature ecology. The
behavior of creatures can make the five elements of external environment lose balance, which can
cause the maladjustment of the creature, then cause a disease. When treating a disease we should
consider the season, behavior living, food and drugs, which are determined by the five elements.
So the process of therapy is that we adjust the internal environment. As a result, if people desire
to be healthy we must protect the natural ecology.
303.
EFFECTS OF OXYGEN ENRICHMENT OF ROOM AIR ON SLEEP STRUCTURE AT HIGH
ALTITUDE . Ha Zhen-De
1
, Jian Xing-Qiong
1
, Zhang Xi-Zhou
1
, Wang Wei
1
, Ma Yong
1
. The
1
8th
Hospital of PLA, Yechen Xinjiang, 8
44
900, China
1
.
Objective: To study the effect of oxygen enrichment of room air on blood oxygen saturation
(SaO2) at an altitude of 3700m. Methods: Raising the oxygen concentration to (24.30±0.88)% in
a room of 4.51m_3.32m_3.41m, twelve men aged 18 to 20 years who had stayed at high altitude
(3700m above sea level) for 30 days slept one night in ambient air and another night in oxygen
enrichment, the order being randomized, their SaO2 were measured in the evening before the
subjects retired to bed and in the next morning and the first, second, third, fourth hour after they
left the dormitory. Results: Mean SaO2 for the first hour in the oxygen enrichment room was
significantly greater than during ambient air in the same room [(94±2.17) % vs. (90.17±3.38) %,
p<0.01], the morning measurement exceeded the evening measurement by an average of 2.33%
after the subjects had slept in oxygen enrichment (p<0.01) and the increase in SaO2 between
evening and morning could persist about 3 hours. Conclusions: Installing an oxygen-enriched
room at high altitude is relatively simple and inexpensive and shows promise for improve sleep
quality, well-being, and work capacity.
304.
THE SDS-PAGE ANALYSIS FOR RECOMBINANTS OF HIF-1alpha; EXPRESSING IN
PLATEAU PIKA. Bai Zhen-Zhong
1
, Yang Ying-Zhong
1
, Ma Lan
1
, Ge Ri-Li
1
. Research Center
for High Altitude Medicine, Qinghai Medical College, Xining, R. P. China
1
.
HIF-1(Hypoxia Induced Factor) monitoring the transcription process of the many relating
genes, it was augmented under the situation of hypoxia. In this essay we choose pika a well-
adapted species living in the Qinghai-Tibetan Plateau as our subjects. To test this, in this essay we
presented the plateau pike for study the HIF-1 expressing in vitro. Aim: We hypothesized that
plateau pika for its' adaptable in high altitude so the increased HIF-1 protein level for molecular
mechanism in the plateau pika living in 4,300m altitude of the Qinghai-Tibetan Plateau. Methods
& Results: The plateau pika captured from Yushu (Qinghai Province) at altitude of 4,300m and
