288.
HIF -1 IN DIFFERENT ORGANS OF HUMAN FETUS AT HIGH ALTITUDE . Cao Yue
1
, Bai
Zheng-Zhong
1
, Ge Ri-Li
1
. Research Center for High Altitude Medicine, Qinghai Medical
College, Xining, P.R.China
1
.
Objectives: RT-PCR was applied to demonstrate the HIF transcriptional differences in
various organs in human fetus living at 4,000 meters and above. Method: Two optimally designed
primers were used according the published human Hypoxia Induced Factor-1 (HIF-1) sequences
from GENEBANK. The total RNA was extracted and the cDNA of HIF-1was obtained by RT-
PCR. Result: 1. A band of 367bp was detected by UV transilluminator from every organs we
tested in agarose gels. 2. The amount of final gene transcription-level of human fetus HIF-1 was
found most in heart, and then kidney, brain, skeleton-muscle, liver, and lungs. Conclusion &
Discussions: 1.The high expression of HIF-1 in heart tissue suggested that the cardiovascular
system is very sensitive to hypoxic condition, which may be protective to the well development
of fetus at the early stage. 2. Expression differentials of HIF-1in varied organs have been laid in
the earlier stage of the human fetus growth. 3. The lungs at fetus stages showed a grayish and
whitish color, which might suggest that they are well adapted to this hypoxic condition in the
womb. 4. Compared with the brain and skeleton muscle, sensing oxygen is blunted in the liver,
which might provide a protective mechanism for the human fetus organs in the hypoxic
conditions. The further study for the mechanism of this order in details should be done in the
future. The work was funded by Natural Sceince Foundation of China (302600354).
289.
HYPOXIC VENTILATORY RESPONSE (HVR) IN HIGH-ALTITUDE PULMONARY
EDEMA (HAPE) SUSCEPTIBLE SUBJECTS AND NEUROTRANSMITTERS. Droma
Yunden
1
, Masayuki Hanaoka
1
, Kazuhisa Urushibata
1
, Toshio Kobayashi
1
, Keishi Kubo
1
.
Departments of Internal Medicine, Shinshu University School of Medicine
1
.
Objective: To demonstrate the role of HVR in the pathogenesis of HAPE; and to figure out
the mechanism of the blunted HVR in HAPE susceptible subjects (HAPE-s) regarding the aspects
of neurotransmitters. Methods: HVR at the 10th minute (min.) and at the 30th min. were
measured in 12 Japanese climbers who had a previous history of HAPE (HAPE-s), and compared
these results with those of 8 otherwise healthy climbers who had no history of HAPE (HAPE-r)
correspondingly. HVR was measured by a progressive isocapnic hypoxic method and was
evaluated by a slope relating minute ventilation to arterial oxygen saturation (_Vmin/_SaO2).
Meanwhile, the peripheral plasma dopamine and L-DOPA were measured under the conditions of
the subjects at normoxia and hypoxia of 30 minutes by a high performance liquid
chromatography (HPLC) method. Results: Results are shown in the table.
HAPE-s
HAPE-r
Normoxia Hypoxia
NormoxiaHypoxia
(_Vmin/_SaO2 (L/min/%) -0.29±0.8*
-1.38±1.24
(at the 10th min.)
_Vmin/_SaO2 (L/min/%)
-0.16±0.11*
-1.29±1.16
(at the 30th min.)
Dopamine (ng/ml)
7.4±3.85 4.58±1.73
6.70±2.58 5.55±1.54
L-DOPA (ng/ml)
2.78±0.49 2.38±0.31§
2.90±0.69 3.06±0.79
*: P < 0.001, compared with HAPE-r by unpaired Student's t test; : P < 0.01, compared with
_Vmin/_SaO2 at the 10th min by paired Student's t test; : P<0.001 compared with Normoxia
within the group by paired Student's t test; §: P < 0.01, compared with HAPE-r under Hypoxia by
unpaired Student's t test. Conclusion: The blunted HVR as well as the persistence of the blunted
HVR along with the duration of hypoxic exposure were demonstrated in HAPE-s. The depression
of the HVR is contributed to the pathogenesis of HAPE. The neurotransmitters in terms of
dopamine and L-DOPA are involved with the mechanisms of the blunted HVR in HAPE-s.
