Compound Codonopsis Pulosula Nanufeldt (Chinese herb; CCPN) developed in our lab in
1970s has been proved an effective protective medicine in hypoxic injury by large amount of
animal tests and on-spot studies. The purpose of this study was to investigate the effect of CCPN
on the hypoxic neuronal apoptosis and elucidate its mechanisms using molecular biological
techniques. Sixty-six Wistar rats (body weight 180-220g) were randomly divided into normoxia
and hypoxic exposure for 2, 4, 6, 8 and 10 days, each group was subdivided into CCPN group and
control group. CCPN were given to the rats by gastric incubation for 7 days before putting into
the hypobaric chamber at a simulated altitude of 7000m for 5 hours each day. Then the rats were
decapitated on the 2nd,4th,6th,8th,10th day of hypoxia, respectively. The result suggests that: 1.
Hypoxia could trigger the internal (mitochondrial) pathway of apoptosis though upregulating
proapoptotic proteins as P53, Bax, Bad, Hsp60 and resulted in apoptosis. 2. CCPN possesses an
antiapoptosis function. There are at least two mechanisms of its action. The first mechanism
involves regulating the expression level of Bcl-2, Bax, Hsp70 and increasing the ratio between
Bcl-2/Bax, which determines apoptosis or survive; while the second one is CCPN upregulates the
Ras associated protein and blocks the internal pathway of apoptosis. Thus, the results indicate that
CCPN exerts its neuroprotective effect on different targets and represents an effective survival
agent with multiple mechanisms of action. * The project supported by National Natural Science
Foundation of China_399790908_
284.
THE CHANGE OF VEGF DURING HYPOXIA AND THE RELATIONSHIP BETWEEN
VEGF AND HAPE. Xie Yin-Zhi
1
, Yang Xi-Ming
1
, Zhang Cui-Ping
1
, Zhang Dong-Xiang
1
, Zhang
Xi-Zhou
2
, Yin Zhao-Yun
1
, Lu Yong-Da
1
. Institute of Health and Environmental Medicine,
Academy of Military Medical Sciences, Tianjin,
1
8th Hospital of PLA;Yecheng;Xinjiang;China
2
.
Objective: To investigate the relationship between the expression of VEGF and the incidence
of high altitude pulmonary edema ( HAPE) and the mechanism of high altitude acclimatization
adaptation. Methods: Pulmonary artery endothelial cells (PAEC) of rat were cultured. The
expression of VEGF in PAEC was detected using RT-PCR and ELISA. Rats were divided
randomly into five groups by weight : control, acute hypoxia (exposed to a simulated 8 000 m
altitude for 4 hours), and three intermittent hypoxia groups ( exposed to a simulated 3 000 m or 5
000 m altitude for 2 weeks, 4 hours a day, then to a simulated 8 000 m altitude for 4 hours) .
VEGF in the lung was detected using immunohistochemistry and slot hybridization, and the
changes of pathology in the lung were observed. VEGF in the blood plasma of rats, in men who
immigrated to high altitude at different times, and in those who suffered from HAPE were
measured using ELISA. Results: The expression of VEGF in PAEC was increased under hypoxia
condition (1 % O2) ( P < 0. 01) . In the blood plasma and lung of rats during hypoxia, levels of
VEGF and VEGF mRNA were higher than that in the control group. VEGF in acute hypoxia
group was increased significantly compared with that in the intermittent hypoxia groups. Leakage
of fluid in the lung of rats was observed in the acute hypoxia group. The longer the rats
acclimatized, the lower the level of VEGF expressed, and the less the fluid leaked in the lung.
The same tendency could be seen in humans. The level of VEGF in patients with HAPE
pretherapy was higher than that measured posttherapy. Conclusions: Hypoxia could stimulate the
expression of VEGF, and the upregulated expression of VEGF is one of the most important
factors of development of HAPE. * The project supported by National Natural Science
Foundation of China (30393130)
285.
THE DIAGNOSTIC VALUE OF MULTICOLORED DOPPLER ULTRASOUND USED IN
INFANTS' HIGH-ALTITUDE HEART DISEASE IN LHASA. Ma Yong-Hong
1
, Wang Yuan-
Sheng
1
, Ouyang Ming
1
. Dept. of Radiology, People's Hospital of Tibet Autonomous Region,
Lhasa
1
.
To discuss the diagnostic value of multicolored Doppler ultrasonic used in high altitude-
induced heart disease for infants. Method: 10 infants with heart diseases were selected from our
hospital to be enrolled into this study. For each subject, a group of 6 parameters of heart sizes and
