281.
EFFECTS OF BRAIN EXTRACT FROM HYPOXIC PRECONDITIONDED MICE ON
PRIMARY CULTURED HIPPOCAMPAL NEURONS. Yan-ling Yin
1
, Jing-jin Li
1
. Dept. of
Neurobiology, Capital University of Medical sciences, Beijing, China
1
.
The present experiment was designed to determine the effect of brain extract (BE) from
hypoxic preconditioned mice on primary cultured hippocampal neurons. Hippocampal neurons
were cultured without serum from the new-borne Wistar rat within 24 hours, then were treated
with 3mmol/L potassium cyanide (KCN) to make emergent hypoxia model. The experiment
were_divided_into_six_ groups: (1) The control; (2) KCN group; (3) Adenosine
preconditioned group (ADO); (4) Adenosine preconditioned and KCN group (ADO+KCN); (5)
BE preconditioned group (BE); (6) BE preconditioned and KCN group (BE+KCN). Apoptotic
neurons were assessed by fluorescent microscopy with Hoechst33342 staining. The neuron
viability was determined by MTT colormetric assay indirectly. BE from hypoxic preconditioned
mice increased neurons viability and reduced the apoptotic rate, which indicated the hypoxic
endurance of neurons was improved. In order to discuss the possible mechanism, spontaneous
excitatory post-synaptic current (sEPSC) of BE preconditioned normal neurons and hypoxic
neurons were recorded by the method of whole-cell patch clamp recording. The amplitude of
sEPSC of group 1-6 were 230.78±13.576pA, 0pA, 157.8±7.771pA, 143.2±2.294pA,
164.72±5.741pA and 142.9±2.874pA, respectively. There was significant difference between
group 1 and 6 (P<0.05); very significant difference between group 2 and 3, 4, 5, 6(P<0.001). The
results of sEPSC's frequency of group1-6 were 409.4±23.592Hz (in 2 min), 0Hz,
224.2±28.273Hz, 122.6±29.374Hz, 293.2±30.092Hz and 293.2±30.092Hzd respectively.
Statistical results showed that there were significant differences between group 1 and 3, 5
(P<0.01), between group 2 and 1,3,4,5(P<0.05). These results suggested that the protecting effect
of BE from hypoxic preconditioned mice are similar to ADO, and the decreasing of cultural cell's
excitability may be involved.
282.
THE CHANGE OF SERUM GASTROINTESTINAL HORMONES IN NORMAL MALES AT
DIFFERENT ALTITUDE. Ma Ying-Cai
1
, Xiong Yuan-zhi
1
, Chu Xing-Qi
1
. Qinghai Provincial
People' Hospital, Xining Qinghai, P.R.China
1
.
The change of serum GAS (Gastrin), MTL (Motilin), PGI (Prostacyclin) and TXA2
(Thromboxane A2) of normal males were studied at different altitudes. Digestive systematic
diseases at high altitude were explored. 62 normal males from three different altitudes were
selected as following: GuangZhou group (2m), Xining group (2260m) and Yanshiping group
(4750m, which is on the line of the Qinghai-Tibetan railway). Their gastrointestinal hormones
were measured by radio-immuno-assay. GAS increased in the Yanshiping group
(62.33ng/L±98.64 ng/L) compared with that in the GuangZhou group(25.63 ng/L±2.79
ng/L)(P<0.05), However, GAS in the other groups did not differ. MTL levels were
430.51pg/ml±515.04 pg/ml, 257.61 pg/ml±155.70 pg/ml and 651.65 pg/ml±638.47 pg/ml,
respectively. These groups did not differ. The PGI of the Yanshiping group (256.79
pg/ml±461.92 pg/ml) was higher than that of the Xining group(78.57 pg/ml±27.86 pg/ml)
(p<0.05), TXA of the Yanshiping group(788.49 pg/ml±470.41 pg/ml) was significantly higher
than that of the Xining group(118.42 pg/ml±66.23 pg/ml) (P<0.001). GAS, MTL, PGI and TXA
in normal males increased with altitude. This indicates that there may be a close relationship
between the adaptation of digestion in humans and digestive system diseases at high altitude.
283.
THE EFFECT OF COMPOUND CODONOPOSIS PILOSULA ON THE CEREBRAL
APOPTOSIS AND ITS MECHANISM AT A SIMULATED 7000M ALTITUDE. Xie Ying-Zhi
1
,
Liu Kan
1
, Zhang Bo
1
, Ma Zi-Min
1
, Fan Ming
2
. (Institute of Hygiene and Environmental
Medicine, Academy of Military Medical Sciences, Tianjin
1
, Institute of Basic Medicine,
Academy of Military Medical Sciences, Beijing, China
2
.
