finally obtained according to the concerned rules in Pharmacopoeia of China. The experimental
results of the hermetic antihypoxia for mice showed that when the dosages of the herbs extracts
prepared with the new technology were as 1/10 as that of the extracts by traditional decocting
technology, its effects of antihypoxia were basically alike with that of the extracts prepared with
traditional method, and significantly better than that of control P<0.05. At the same time, the
results verified in army officials and soldiers on the high altitude location also confirmed that
when the oral dosages of the new form of FDD were as 1/6 as that of FDP, its effects of
preventing and treating the acute mountain sickness were still better than that of FDP, and was
notably better than that of control P<0.05. Thus it can be seen that the technologies of extracting
effective components from the herbs with the microwave at low temperature and preparing FDD
are the advantages of shorter productive period, less energy consumption, easy to operate, lower
costs and stable conditions. Similarly, FDD possesses the characteristics of novelty preparation,
lower dosages, rapid effects, notable results of prevention and treatment for the acute mountain
sickness, no sugar, no toxic and side effects, easy to be taken and carried etc., so it may be
hopeful to substitute it for FDP in the future.
277.
ANALYSIS OF NITRIC OXIDE LEVELS IN NORMAL SUBJECTS AT DIFFERENT
ALTITUDES. Yang Yan-Qing
1
, Wang Xiang-Yong
1
, Ren Wei-Quan
1
, Liu Lan-Min
1
, Ji Bing
1
,
Yu Li-Hua
1
. Dept. of Laboratory, Qinghai Provincial People's Hospital, Xining, Qinghai,
P.R.China
1
.
Nitric Oxide (NO) is a fat-soluble gas. It has extensive function among cells. NO may be an
endothelium derived relaxing factor (EDRF) according to known publications in biochemistry
and pharmacology. Most animal experiments have indicated that basal excretion of NO in animal
lungs plays an important role in maintaining low tension in pulmonary vessels. In different
animals, different inhibitors of NO synthesis can strengthen contraction of pulmonary vessels to
respond to acute hypoxia in different animals. Our findings indicate that decreasing levels of NO
secondary to hypoxia causes pulmonary vessel contraction. Long-term hypoxia may cause a
change in the synthesis of NO synthase (NOS) in endodermis cells, which results in decreased
levels of NO. It is necessary to measure the serum concentration of NO in 210 normal subjects at
different altitudes. No subjects had cardiac or pulmonary disease. Subjects were divided into five
groups, according to altitude: 50 samples in Beijing50m, 123.6±27.6umol/L, 60 samples in
Xining (2260m,114.3±28.3umol/L), 37 samples in Geermu (2800m,110.3±19.2umol/L), 31
samples in Wutumeiren (3100m,96.3±13.6umol/L), 32 samples in Yanshiping
(4750m,89.3±13.2umol/L). All samples were tested by the nitrate reductase method. Obvious
differences existed in two of the groups. Results indicate that serum NO concentrations decrease
with increasing altitude (p <0.05-0.01). This phenomenon is somehow related to hypoxia, which
results in pulmonary vessel contraction. A possible mechanism for this reaction could involve
NO-induced intracellular cGMP (cyclic guanosine monophosphate), increasing GC (guanylate
cyclase), and causing vasodilation.
278.
EFFECTS OF TIBETAN DRUGS ON CHANGES OF SAO
2
IN YOUNG MEN AFTER STEP-
TEST AT HIGH ALTITUDE . Wang Yi
1
, Niu Wen-Zhong
1
, Li Ming
1
, Zhang Chang-Fa
1
. The
center of disease prevention and control of Chengdu Military Command, Chengdu, China
1
.
Objective: To seek for a new method against hypoxia, the effects of two new compound
Tibetan drugs on changes in SaO2 of young men at high altitude were observed. Method: 96
subjects were divided randomly into three groups, and they were given Luobusang capsule, Di'ao
Xinxuekang capsule and placebo, respectively. A single blind procedure was used. Before three
days and after five days of being at high altitude (3680 m), they took different drugs according to
their group, 2 capsules each time, three times a day for 8 days. After they entered high altitude 5
days, their resting SaO2 and SaO2 during the recovery phase after step-test(step height 40cmm
and 25step/min) 0min,1min,2min,3min,5min were measured, respectively. Result: Compared
with before stepping, there were significant decrease in SaO2 during the recovery phase in
