Integrating in
Dream'lineTM
the powerful
proprietary
technologies,
EvosightTM and L-ShufflingTM
,
Proteus offers you a outstanding opportunity to
develop tailor-made proteins
via unique and
flexible services.
Rapidly evaluate in parallel a very large panel of
cell-based and cell-free expression systems
and conditions
for your genes.
Obtain proteins in
scaleable production system
from research scale toward process development.
Dramatic 50-fold improvement of the activity of a protein using a combination of
EvoSightTM and L-ShufflingTM. The figure shows the actual distribution of the activity
of the clones generated by the third round of L-ShufflingTM compared with the
starting enzyme (wild type : "WT"). Most clones exhibit an activity higher than the
original WT enzyme.
CONTACT PSI : Emmanuel MAILLE
Parc Georges Besse - 70, allée Graham Bell
30000 - Nimes France
Tel: +33(0)466 70 64 67 - Fax: +33(0)466 70 64 60
E-mail : psi@proteus.fr
Dream'line
T M
: Directed Evolution Services
Prod'line
T M
: Protein Production Services
Cell-based
expression
Your genes
Cell-free
expression
EXPRESSION PROFILING
An amazing panel
of more than 200 expression
systems and conditions
Cell-based small
scale production
Cell-free
customized kits
LAB SCALE PRODUCTION
SCALE-UP TOWARD INDUSTRIALIZATION
Fermentation, downstream processing,
purification, QC
L-Shuffling
TM
Random genetic
recombination
IMPROVED VARIANTS
Single gene
Evosight
TM
Reasoned random
mutagenesis
OPTIMAL
LIBRARY OF
MUTANT GENES
Family of genes
Parental genes
Rapidly create and optimize novel proteins
0
10
20
30
40
50
R
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l
a
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A
c
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i
v
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2nd round of
L-Shuffling
TM
3rd round of L-Shuffling
TM
50-fold increase
1st round of
L-Shuffling
TM
WT
EvoSight
TM
