Pepscan Systems Saint Pepscan Page 2
Transfection efficiency of cell-types:
At Synvolux Therapeutics we developed
and optimized a different, non toxic high
efficient, protocol which is unique
compared to other transfection reagent
protocols.
To the right you see a small overview of
some cell-lines however, at our website
you will find more information about
different cell-types and some protocols
which have been used to transfect these
cell-lines.
cell line
% in 24h
% in 48h
COS-7
80
HEK293
80
Cho-K1
71,1
B16-F10
70
3T3
65
ECV304
53,2
Hela
50
H5V
46
SAOS-2
38
MCF-7
35
RCC4
32,3
Phoenix
32
C26
26
GLC-45
22
SW948
18
P19
14
HEP-G2
10,5
FLF
10
HUAEC
9,8
GLC-4
8
Ts20b
4,5
HUVEC
4,4
H38-5
2
Stability of the SAINT-DNA complex:
Without serum added to the complex
In the presence of serum
- Black bars are B16 cells.
- With bars are Hek 293 cells
0
10
20
30
40
50
60
70
80
90
100
Percenta ge of GF P exp ressing ce lls
C O S-7*
H EK 2 93*
C H O -K1*
B 16- F1 0*
3 T 3*
E C V3 04*
H E LA
H 5V*
SAO S-2 *
M C F- 7
R C C 4*
Ph oen ix
C 26 *
G LC -4 5
S W 9 48
P 19
H E P-G 2
F L F*
H U A EC
GLC -4
Ts2 0b
H U VEC
H 38 -5
0
10
20
30
40
50
60
70
0
5
10
15
20
30
Time complex formation (minutes)
% GFP positive cells
0
10
20
30
40
50
60
0
5
10
15
20
30
Time complex formation (minutes)
