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American Journal of Ophthalmology - AJO 02 333 R 2 (Page 3)

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American Journal of Ophthalmology - AJO 02 333 R 2
The retrospective nature of this study did not permit us to establish a clear and
reproducible research protocol. This is a pilot study and it is by no means a thorough
evaluation of the use of trypan blue in vitreoretinal surgery.

In conclusion, visualisation and dissection of the ILM was facilitated by trypan blue
staining,visual acuity was greatly improved and no signs of toxicity or adverses reactions
were noted after up to 1 year of follow-up.


1)Gardorfer A, Haritoglou C, Gass CA, Ulbig MW, Kampik A. Indocyanine green-
assisted peeling of the internal limiting membrane may cause retinal damage. Am J
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2)Engelbrecht N, Freeman J, Sternberg P, Aaberg T. Retinal pigment epithelial changes
after macular hole surgery with indocyanine green-assisted internal limiting membrane
peeling. Am J Ophthalmolol. 2002 Jan; 133 (1) :89-94

3)Schmidt KL, Milner K, Hilburn PJ, Schmidt WA. Ultrastructure of trypan blue
induced ocular defects : I. Retina and lens. Teratology. 1983 Aug; 28 (1): 131-44

4)Norn MS. Per operative trypan blue vital staining of corneal endothelium. Eight years'
follow up. Acta Ophthalmol. 1980; 58: 550-5

5)Veckeneer M, van Overdam K, Monzer J, et al. Ocular toxicity study of trypan blue
injected into the vitreous cavity of rabbit eyes. Graefes Arch Clin Exp Ophthalmol.
2001; 239: 698-704

6)Feron E, Veckeneer M, Parys-Van Ginderdeuren R,et al. Trypan blue staining of
epiretinal membranes in proliferative vitreoretinopathy. Arch Opthalmol. 2002; 120(2):

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