14
Downstream
thirty seven
Adsorbent STREAMLINE Direct CST I
Characteristics of STREAMLINE Direct CST I
Type of exchanger
High-salt tolerant
Functional group
Multi-modal function
Matrix structure
Macroporous cross-linked 4% agarose
containing stainless steel core material
Particle form
Spherical, 80 to 165 µm
Mean particle size
135 µm
Mean particle density
1.8 g/ml
Degree of expansion
3.0
(H/H
o
) at 900 cm/h
pH stability
working range
3 13
cleaning range
2 13
Chemical stability
All commonly used aqueous buffers.
1 M NaOH
20% ethanol
Avoid
Organic solvents
Oxidizing agents
Physical stability
Generation of fines during normal operation is
negligible. Avoid handling drained adsorbent in
shearing operations.
Binding capacity*
30 mg BSA/ml adsorbent
Storage
20% ethanol
* Breakthrough capacity of 2 mg/ml bovine serum albumin in packed bed mode at 600 cm/h in
50 mM acetate buffer, pH 4.5. Conductivity 28 mS, bed height 20 cm.
STREAMLINE portfolio
STREAMLINE products, know-how and success have grown
steadily over the years. The product portfolio comprises
adsorbents, columns, systems and support. In addition to
the new Direct products, standard STREAMLINE adsorbents
cover affinity and ion exchange chromatography. Other
adsorbents are available through out CDM service.
Systems and support
STREAMLINE systems are available in a range of sizes and
configurations suitable for processes in GMP environments.
The systems feature fully automated operation, gradient
formation, UNICORNTM control system or PLC automation.
Applications know-how, validation support and an extensive
library of technical information and references are available
to smooth your pathway to production. Installation and
Operational Qualification Documentation Packages, for
example, help get STREAMLINE hardware up and running
in accordance with regulatory needs.
For more information about STREAMLINE Direct, ask for:
Data File: STREAMLINE Direct CST I
18-1177-65
Data File: STREAMLINE 24 and 95
18-1177-63
Application Note: Sanitization of STREAMLINE Direct 95 column 11-0007-39
STREAMLINE Direct columns and adsorbent
New columns currently available are STREAMLINE Direct 24 (i.d 24 mm) and STREAMLINE Direct 95 (i.d 95 mm) for process
development and pilot-scale, respectively. Production columns STREAMLINE Direct 280 and STREAMLINE Direct 600 will be
introduced shortly. For more details, please contact your local representative.
Primary column specifications.
Column
Inner diameter
Tube height
Max working
Total height
1
Footprint
2
Max working
Working
(mm)
(mm)
height (mm)
(mm)
(mm)
pressure (bar)
temperature (°C)
STREAMLINE Direct 24/1.7
24
1700
1595
2 200
348x363
5
+4 to +40
STREAMLINE Direct 95/1.0
95
1000
997
1 550
348x363
1
+4 to +40
STREAMLINE Direct 95/1.7
95
1700
1697
2 250
348x363
1
+4 to +40
1 The height includes outlet tubing on the top of the column.
2 The footprint includes the control unit
Working characteristics and recommended fluid velocities
Column
Sedimented
Sedimented
Recommended
Fluid velocity range
Fluid velocity range
bed height (cm)
adsorbent(L)
max expanded
for STREAMLINE Direct
other STREAMLINE
min max
1
min max
1
bed height
1
(cm)
Adsorbent
2
(cm/h)
Adsorbents
3
(cm/h)
STREAMLINE Direct 24/1.7
20 50
0.090 0.226
150
400 800
200 500
STREAMLINE Direct 95/1.0
20 27
1.42 1.91
80
400 800
200 500
STREAMLINE Direct 95/1.7
20 50
1.42 3.54
150
400 800
200 500
1 The recommended max bed heights can be exceeded if the bed surface at the top of the expanded bed is visible
2 Mean density 1.8 g/ml
3 Mean density 1.2 to 1.3 g/ml
www.bioprocess.amershambiosciences.com
Under menu: Products
Chromatography media
Expanded bed adsorption,
or under menu: Products
Chromatography columns
STREAMLINE columns.
Or contact your local representative.
